AP09246PU-N

antibody from Acris Antibodies GmbH

Targeting: FANCG FAG, XRCC9

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09246PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09246PU-N, RRID:AB_2036114

Product name

anti XRCC9 / FANCG (1-12)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 1-12 of human FANCG protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.93 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot using anti-FANCG antibody shows detection of a band at ~69 kDa (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate. Approximately 35 μg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Immunohistochemistry. Anti-FANCG antibody shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.