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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Immunohistochemistry [2]
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- Product number
- 37-0600 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Cyclophilin B Monoclonal Antibody (4E11G1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4E11G1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- -20°C
Submitted references Cyclophilin B as a co-regulator of prolactin-induced gene expression and function in breast cancer cells.
Expression of cyclophilin B is associated with malignant progression and regulation of genes implicated in the pathogenesis of breast cancer.
The isolation of novel mesenchymal stromal cell chemotactic factors from the conditioned medium of tumor cells.
Fang F, Zheng J, Galbaugh TL, Fiorillo AA, Hjort EE, Zeng X, Clevenger CV
Journal of molecular endocrinology 2010 Jun;44(6):319-29
Journal of molecular endocrinology 2010 Jun;44(6):319-29
Expression of cyclophilin B is associated with malignant progression and regulation of genes implicated in the pathogenesis of breast cancer.
Fang F, Flegler AJ, Du P, Lin S, Clevenger CV
The American journal of pathology 2009 Jan;174(1):297-308
The American journal of pathology 2009 Jan;174(1):297-308
The isolation of novel mesenchymal stromal cell chemotactic factors from the conditioned medium of tumor cells.
Lin SY, Yang J, Everett AD, Clevenger CV, Koneru M, Mishra PJ, Kamen B, Banerjee D, Glod J
Experimental cell research 2008 Oct 15;314(17):3107-17
Experimental cell research 2008 Oct 15;314(17):3107-17
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cyclophilin B showing staining in the cytoplasm and weak staining in the nucleus of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclophilin B Mouse Monoclonal Antibody (Product # 37-0600) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cyclophilin B showing staining in the cytoplasm of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclophilin B Mouse Monoclonal Antibody (Product # 37-0600) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
