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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-24736 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HINT1 Monoclonal Antibody (1500CT836.13.93)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1500CT836.13.93
- Vial size
- 400 µL
- Concentration
- 0.45 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HINT1 in U-2 OS cells. Fixation was performed with 4% paraformaldehyde and the cells were permeabilized with 0.1% Triton X-100. Sample was probed with a HINT1 monoclonal antibody (Product # MA5-24736) using a dilution of 1:25, followed by Dylight 488-conjugated goat anti-mouse IgG using a dilution of 1:200. Dylight 554 (1:100) and DAPI were used to counterstain cytoplasmic actin and cell nuclei, respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human kidney tissue. Fixation was performed with formaldehyde, blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Sample was probed for 1 hours at 37 C with a HINT1 monoclonal antibody (Product # ) with a dilution of 1:25. Detection was performed with polyvalent goat biotinylated secondary antibody.
