PA5-60760

antibody from Invitrogen Antibodies

Targeting: HINT1 HINT, PKCI-1, PRKCNH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-60760

Provider

Invitrogen Antibodies

Product name

HINT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: IPKKHISQIS VAEDDDESLL GHLMIVGKKC AADLGLNKGY RMVVNEGSDG GQSVYHVHLH VL

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HINT1 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a HINT1 Polyclonal Antibody (Product # PA5-60760).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HINT1 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a HINT1 Polyclonal Antibody (Product # PA5-60760).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HINT1 in human cell line U-251 MG shows positivity in plasma membrane & cytoplasm. Samples were probed using a HINT1 Polyclonal Antibody (Product # PA5-60760).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HINT1 in human gastrointestinal using HINT1 Polyclonal Antibody (Product # PA5-60760) shows cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HINT1 in human cerebral cortex using HINT1 Polyclonal Antibody (Product # PA5-60760) shows moderate positivity in neuropil.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HINT1 in human kidney using HINT1 Polyclonal Antibody (Product # PA5-60760) shows moderate cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HINT1 in human fallopian tube using HINT1 Polyclonal Antibody (Product # PA5-60760) shows moderate cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HINT1 in human thyroid gland using HINT1 Polyclonal Antibody (Product # PA5-60760) shows moderate cytoplasmic and nuclear positivity in glandular cells.