Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-27603 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: K562, THP-1, HL-60, U87-MG, SK-N-SH, IMR32, rat testis.
- Concentration
- 0.66 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM27 in non-transfected lysate 293T lysate (A) and transfected lysate (B). Samples were resolved on 7.5% SDS PAGE gel and probed using a TRIM27 polyclonal antibody (Product # PA5-27603) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIM27 Polyclonal Antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 Polyclonal Antibody (Product # PA5-27603) diluted by 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRIM27 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TRIM27 Polyclonal Antibody (Product # PA5-27603) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TRIM27 Polyclonal Antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 Polyclonal Antibody (Product # PA5-27603) diluted by 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRIM27 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TRIM27 Polyclonal Antibody (Product # PA5-27603) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRIM27 in paraformaldehyde-fixed HeLa cells using a TRIM27 polyclonal antibody (Product # PA5-27603) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of TRIM27 was performed in Jurkat whole cell extracts using 5 µg of TRIM27 Polyclonal Antibody (Product # PA5-27603). Samples were transferred to a membrane and probed with TRIM27 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.