Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30099 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFAB1 Polyclonal Antibody (Product # PA5-30099). Sample (30 µg of whole cell lysate). Lane A: 293T. 12% SDS PAGE. NDUFAB1 Polyclonal Antibody (Product # PA5-30099) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFAB1 Polyclonal Antibody detects NDUFAB1 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.12 % SDS-PAGE. NDUFAB1 Polyclonal Antibody (Product # PA5-30099) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFAB1 Polyclonal Antibody detects NDUFAB1 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.12 % SDS-PAGE. NDUFAB1 Polyclonal Antibody (Product # PA5-30099) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NDUFAB1 showing staining in the cytoplasm of A431 cells. A431 cells were fixed in ice-cold MeOH for 5 min and stained using a NDUFAB1 polyclonal antibody (Product # PA5-30099) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFAB1 Polyclonal Antibody detects NDUFAB1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: NDUFAB1 protein stained by NDUFAB1 Polyclonal Antibody (Product # PA5-30099) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NDUFAB1 (Product # PA5-30099) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.