Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-59110 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MFF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MFF Antibody [NBP2-59110] - Detection of human and mouse MFF by western blot. Samples: Whole cell lysate from HeLa (5 and 15 ug) 293T, (50ug), and Jurkat (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MFF antibody NBP2-59110 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MFF Antibody [NBP2-59110] - Detection of human MFF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T (50ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MFF antibody NBP2-59110 used for IP at 20 ul per reaction. MFF was also immunoprecipitated by rabbit anti-MFF antibody NBP2-59109. For blotting immunoprecipitated MFF,NBP2-59110 was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.