Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-48258 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PALB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PALB2 in HepG2 cell line lysates (35 µg/lane). Lysates were probed with a PALB2 Antibody (Center) (Product # PA5-48258).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Partner and localizer of BRCA2 was achieved by transfecting MCF7 cells with Partner and localizer of BRCA2 specific siRNA (Silencer® select Product # S527981, S36200). Immunofluorescence analysis was performed on untransfected MCF7 cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Partner and localizer of BRCA2 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with PALB2 Polyclonal Antibody (Product # PA5-48258, 1:100 dilution) followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300 dilution) was used for cytoskeletal F-actin (Red) staining. Reduction of the specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Partner and localizer of BRCA2. The Images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PALB2 in HepG2 cells (right histogram) compared to a negative control cell (left histogram). Cells were probed with a PALB2 Antibody (Center) (Product # PA5-48258). Following incubation with the primary antibody, cells were incubated with FITC-conjugated goat-anti-rabbit secondary antibodies.