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- Antibody Data
- Antigen structure
- References [14]
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- Immunohistochemistry [1]
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- Product number
- M00059 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Smad3 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for SMAD3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat SMAD3 information: Molecular Weight: 48081 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- FG-19
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1.
GGTLC1 knockdown inhibits the progression of endometrial cancer by regulating the TGF-β/Smad signaling pathway.
TSLP Induces Epithelial-Mesenchymal Transition in Nasal Epithelial Cells From Allergic Rhinitis Patients Through TGF-β1/Smad2/3 Signaling.
Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.
Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper.
Enhanced transforming growth factor-beta signaling and fibrosis in the pectoralis major muscle of broiler chickens affected by wooden breast myopathy.
Mechanistic studies on the role of TGF-β1 in angiogenesis through EndMT.
Semaphorin 7A promotes endothelial to mesenchymal transition through ATF3 mediated TGF-β2/Smad signaling.
Reciprocal enhancement of thrombosis by endothelial-to-mesenchymal transition induced by iliac vein compression.
Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway.
Sodium humate accelerates cutaneous wound healing by activating TGF-β/Smads signaling pathway in rats.
Molecular responses of radiation-induced liver damage in rats.
Dexamethasone attenuates bleomycin-induced lung fibrosis in mice through TGF-β, Smad3 and JAK-STAT pathway.
Pathogenesis pathways of idiopathic pulmonary fibrosis in bleomycin-induced lung injury model in mice.
Zhao J, Liu X, Jin X, Dong T, Gao X, Wang J, Li Y, Ma E
Bioorganic chemistry 2024 May;146:107274
Bioorganic chemistry 2024 May;146:107274
GGTLC1 knockdown inhibits the progression of endometrial cancer by regulating the TGF-β/Smad signaling pathway.
Tang X, Bi X, Yang A, Wang Q, Yang Y
Heliyon 2024 Jun 15;10(11):e31973
Heliyon 2024 Jun 15;10(11):e31973
TSLP Induces Epithelial-Mesenchymal Transition in Nasal Epithelial Cells From Allergic Rhinitis Patients Through TGF-β1/Smad2/3 Signaling.
Yu HW, Wang WW, Jing Q, Pan YL
American journal of rhinology & allergy 2023 Nov;37(6):739-750
American journal of rhinology & allergy 2023 Nov;37(6):739-750
Endosulfan induced kidney cell injury by modulating ACE2 through up-regulating miR-429 in HK-2 cells.
Zhang B, Liu S, Sun Y, Xu D
Toxicology 2023 Jan 15;484:153392
Toxicology 2023 Jan 15;484:153392
Differential expression of microRNAs in human endometrium after implantation of an intrauterine contraceptive device containing copper.
Fan X, Huang J, Xu C, Bao M, Xia W, Zhu C
Molecular human reproduction 2021 Sep 1;27(9)
Molecular human reproduction 2021 Sep 1;27(9)
Enhanced transforming growth factor-beta signaling and fibrosis in the pectoralis major muscle of broiler chickens affected by wooden breast myopathy.
Xing T, Zhao ZR, Zhao X, Xu XL, Zhang L, Gao F
Poultry science 2021 Mar;100(3):100804
Poultry science 2021 Mar;100(3):100804
Mechanistic studies on the role of TGF-β1 in angiogenesis through EndMT.
Cao SJ, Hong L, Li XQ
Vascular 2021 Jun;29(3):442-450
Vascular 2021 Jun;29(3):442-450
Semaphorin 7A promotes endothelial to mesenchymal transition through ATF3 mediated TGF-β2/Smad signaling.
Hong L, Li F, Tang C, Li L, Sun L, Li X, Zhu L
Cell death & disease 2020 Aug 10;11(8):695
Cell death & disease 2020 Aug 10;11(8):695
Reciprocal enhancement of thrombosis by endothelial-to-mesenchymal transition induced by iliac vein compression.
Hong L, Du X, You T, Sun L, Li W, Xiao L, Lu H, Wang W, Li X
Life sciences 2019 Sep 15;233:116659
Life sciences 2019 Sep 15;233:116659
Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway.
Wang W, Zha G, Zou JJ, Wang X, Li CN, Wu XJ
Current medical science 2019 Oct;39(5):748-753
Current medical science 2019 Oct;39(5):748-753
Sodium humate accelerates cutaneous wound healing by activating TGF-β/Smads signaling pathway in rats.
Ji Y, Zhang A, Chen X, Che X, Zhou K, Wang Z
Acta pharmaceutica Sinica. B 2016 Mar;6(2):132-40
Acta pharmaceutica Sinica. B 2016 Mar;6(2):132-40
Molecular responses of radiation-induced liver damage in rats.
Cheng W, Xiao L, Ainiwaer A, Wang Y, Wu G, Mao R, Yang Y, Bao Y
Molecular medicine reports 2015 Apr;11(4):2592-600
Molecular medicine reports 2015 Apr;11(4):2592-600
Dexamethasone attenuates bleomycin-induced lung fibrosis in mice through TGF-β, Smad3 and JAK-STAT pathway.
Shi K, Jiang J, Ma T, Xie J, Duan L, Chen R, Song P, Yu Z, Liu C, Zhu Q, Zheng J
International journal of clinical and experimental medicine 2014;7(9):2645-50
International journal of clinical and experimental medicine 2014;7(9):2645-50
Pathogenesis pathways of idiopathic pulmonary fibrosis in bleomycin-induced lung injury model in mice.
Shi K, Jiang J, Ma T, Xie J, Duan L, Chen R, Song P, Yu Z, Liu C, Zhu Q, Zheng J
Respiratory physiology & neurobiology 2014 Jan 1;190:113-7
Respiratory physiology & neurobiology 2014 Jan 1;190:113-7
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse kidney, using Smad3 Antibody(M00059)SMAD3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD3 Antibody (M00059)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
