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- Antigen structure
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- Immunohistochemistry [1]
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- Product number
- AF3086 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LINGO-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LINGO-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human LINGO-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_116197- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength.
Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
The Journal of investigative dermatology 2010 Jun;130(6):1685-96
The Journal of investigative dermatology 2010 Jun;130(6):1685-96
Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength.
Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 12;28(11):2753-65
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 12;28(11):2753-65
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- LINGO-1 in Mouse Spinal Cord. LINGO-1 was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human LINGO-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3086) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
