Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-28873 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLF10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa nuclear, eGFP-human KLF10-transfected 293T.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF10 Polyclonal Antibody detects KLF10 protein by western blot analysis. A. 30 µg 293T. B. 30 µg of eGFP-human KLF10-transfected 293T cells.7.5% SDS-PAGE. KLF10 Polyclonal Antibody (Product # PA5-28873) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF10 Polyclonal Antibody detects KLF10 protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract. B. 30 µg HeLa nuclear lysate/extract.10% SDS-PAGE. KLF10 Polyclonal Antibody (Product # PA5-28873) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KLF10 Polyclonal Antibody immunoprecipitates KLF10 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract. A. Control with 2 µg of preimmune rabbit IgG. B. Immunoprecipitation of KLF10 protein by 2 µg of KLF10 Polyclonal Antibody (Product # PA5-28873) 7.5% SDS-PAGE. The immunoprecipitated KLF10 protein was detected by KLF10 Polyclonal Antibody (Product # PA5-28873) diluted at 1:1,000.