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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NB100-604 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-604, RRID:AB_2299970
- Product name
- Rabbit Polyclonal PNUTS/PPP1R10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Upregulation of Phosphatase 1 Nuclear-Targeting Subunit (PNUTS) Is an Independent Predictor of Poor Prognosis in Prostate Cancer.
Marx A, Luebke AM, Clauditz TS, Steurer S, Fraune C, Hube-Magg C, Büscheck F, Höflmayer D, Tsourlakis MC, Möller-Koop C, Simon R, Sauter G, Göbel C, Lebok P, Dum D, Kind S, Minner S, Izbicki J, Schlomm T, Huland H, Heinzer H, Burandt E, Haese A, Graefen M, Meiners J
Disease markers 2020;2020:7050146
Disease markers 2020;2020:7050146
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of Human PNUTS on HeLa whole cell lysates using NB100-604. IP of PNUTS was successfully performed with NB100-603, NB100-605, and another PNUTS polyclonal. IPed PNUTS was blotted using NB100-605.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of Human PNUTS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PNUTS antibody NB100-604 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNUTS antibody NB100-604 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PNUTS antibody NB100-604 (lot NB100-604-3) used for IP at 3 ug per reaction. PNUTS was also immunoprecipitated by a previous lot of this antibody (lot NB100-604-2) and rabbit anti-PNUTS antibody NB100-605. For blotting immunoprecipitated PNUTS, NB100-604 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PNUTS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PNUTS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PNUTS/PPP1R10 Antibody [NB100-604] - Detection of human PNUTS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PNUTS (NB100-604). Detection: DAB
