Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
Reference
Comment
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- Product number
- H00055210-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00055210-D01, RRID:AB_10718149
- Product name
- ATAD3A MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human ATAD3A protein.
- Antigen sequence
MSWLFGINKGPKGEGAGPPPPLPPAQPGAEGGGDR
GLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRY
AKDALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKS
EQIRAQAEERRKTLSEETRQHQARAQYQDKLARQR
YEDQLKQQQLLNEENLRKQEESVQKQEAMRRATVE
REMELRHKNEMLRVEAEARARAKAERENADIIREQ
IRLKAAEHRQTVLESIRTAGTLFGEGFRAFVTDWD
KVTATVAGLTLLAVGVYSAKNATLVAGRFIEARLG
KPSLVRETSRITVLEALRHPIQVSRRLLSRPQDAL
EGVVLSPSLEARVRDIAIATRNTKKNRSLYRNILM
YGPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMG
REGVTAMHKLFDWANTSRRGLLLFVDEADAFLRKR
ATEKISEDLRATLNAFLYRTGQHSNKFMLVLASNQ
PEQFDWAINDRINEMVHFDLPGQEERERLVRMYFD
KYVLKPATEGKQRLKLAQFDYGRKCSEVARLTEGM
SGREIAQLAVSWQATAYASEDGVLTEAMMDTRVQD
AVQQHQQKMCWLKAEGPGRGDEPSPS- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Atad3 function is essential for early post-implantation development in the mouse.
Goller T, Seibold UK, Kremmer E, Voos W, Kolanus W
PloS one 2013;8(1):e54799
PloS one 2013;8(1):e54799
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ATAD3A expression in transfected 293T cell line (H00055210-T01) by ATAD3A MaxPab polyclonal antibody.Lane 1: ATAD3A transfected lysate(66.20 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ATAD3A MaxPab rabbit polyclonal antibody. Western Blot analysis of ATAD3A expression in HeLa.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to ATAD3A on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATAD3A transfected lysate using anti-ATAD3A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ATAD3A purified MaxPab mouse polyclonal antibody (B01P) (H00055210-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol