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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- HPA005680 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA005680, RRID:AB_2667388
- Product name
- Anti-ANLN
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
IVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGG
TGIKPFLERFGERCQEHSKESPARSTPHRTPIITP
NTKAIQERLFKQDTSSSTTHLAQQLKQERQKELAC
LRGRFDKGNIWSAEKGGNSKSKQLETKQETH- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references Systematic antibody generation and validation via tissue microarray technology leading to identification of a novel protein prognostic panel in breast cancer
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
O´Leary P, Penny S, Dolan R, Kelly C, Madden S, Rexhepaj E, Brennan D, McCann A, Pontén F, Uhlén M, Zagozdzon R, Duffy M, Kell M, Jirström K, Gallagher W
BMC Cancer 2013 ;13(1):175
BMC Cancer 2013 ;13(1):175
Generation of monospecific antibodies based on affinity capture of polyclonal antibodies
Hjelm B, Forsström B, Igel U, Johannesson H, Stadler C, Lundberg E, Ponten F, Sjöberg A, Rockberg J, Schwenk J, Nilsson P, Johansson C, Uhlén M
Protein Science 2011 November;20(11):1824-1835
Protein Science 2011 November;20(11):1824-1835
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis in human cell line U-251 MG.
Enhanced validation
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and skeletal muscle tissues using Anti-ANLN antibody. Corresponding ANLN RNA-seq data are presented for the same tissues.
- Sample type
- HUMAN
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows high expression.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human skeletal muscle shows low expression as expected.
- Sample type
- HUMAN
