10639-1-AP

antibody from Invitrogen Antibodies

Targeting: PACSIN3 SDPIII

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

10639-1-AP

Provider

Invitrogen Antibodies

Product name

PACSIN3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAPEEDAGG EALGGSFWEA GNYRRTVQRV EDGHRLCGDL VSCFQERARI EKAYAQQLAD WARKWRGTVE KGPQYGTLEK AWHAFFTAAE RLSALHLEVR EKLQGQDSER VRAWQRGAFH RPVLGGFRES RAAEDGFRKA QKPWLKRLKE VEASKKSYHA ARKDEKTAQT RESHAKADSA VSQEQLRKLQ ERVERCAKEA EKTKAQYEQT LAELHRYTPR YMEDMEQAFE TCQAAERQRL LFFKDMLLTL HQHLDLSSSE KFHELHRDLH QGIEAASDEE DLRWWRSTHG PGMAMNWPQF E (1-300 aa encoded by BC007914)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.32 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 10639-1-AP (PACSIN3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 10639-1-AP (PACSIN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 10639-1-AP (PACSIN3 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 10639-1-AP (PACSIN3 antibody) at dilution of 1:100 (under 25x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 MCF-7 cells were stained with 0.2ug PACSIN3 antibody (10639-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.