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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- GTX83687 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83687, RRID:AB_10728075
- Product name
- SAMHD1 antibody [1F6]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
Submitted references Sulforaphane Inhibits HIV Infection of Macrophages through Nrf2.
Cullin4A and cullin4B are interchangeable for HIV Vpr and Vpx action through the CRL4 ubiquitin ligase complex.
HIV relies on neddylation for ubiquitin ligase-mediated functions.
Furuya AK, Sharifi HJ, Jellinger RM, Cristofano P, Shi B, de Noronha CM
PLoS pathogens 2016 Apr;12(4):e1005581
PLoS pathogens 2016 Apr;12(4):e1005581
Cullin4A and cullin4B are interchangeable for HIV Vpr and Vpx action through the CRL4 ubiquitin ligase complex.
Sharifi HJ, Furuya AK, Jellinger RM, Nekorchuk MD, de Noronha CM
Journal of virology 2014 Jun;88(12):6944-58
Journal of virology 2014 Jun;88(12):6944-58
HIV relies on neddylation for ubiquitin ligase-mediated functions.
Nekorchuk MD, Sharifi HJ, Furuya AK, Jellinger R, de Noronha CM
Retrovirology 2013 Nov 18;10:138
Retrovirology 2013 Nov 18;10:138
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-SAMHD1 mouse monoclonal antibody (GTX83687) immunofluorescent staining of COS7 cells transiently transfected with SAMHD1
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206013 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SAMHD1 antibody(GTX83687), and then analyzed by flow cytometry.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody(GTX83687),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
