12586-1-AP
antibody from Invitrogen Antibodies
Targeting: SAMHD1
AGS5, HDDC1, Mg11, MOP-5, SBBI88
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [2]
- Other assay [1]
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Validation data
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- Product number
- 12586-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAMHD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MQRADSEQP SKRPRCDDSP RTPSNTPSAE ADWSPGLELH PDYKTWGPEQ VCSFLRRGGF EEPVLLKNIR ENEITGALLP CLDESRFENL GVSSLGERKK LLSYIQRLVQ IHVDTMKVIN DPIHGHIELH PLLVRIIDTP QFQRLRYIKQ LGGGYYVFPG ASHNRFEHSL GVGYLAGCLV HALGEKQPEL QISERDVLCV QIAGLCHDLG HGPFSHMFDG RFIPLARPEV KWTHEQGSVM MFEHLINSNG IKPVMEQYGL IPEEDICFIK EQIVGPLESP VEDSLWPYKG RPENKSFLYE IVSNKRNGID VDKWD (1-314 aa encoded by BC036450)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- L02 cells were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 12586-1-AP (SAMHD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 12586-1-AP (SAMHD1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12586-1-AP ( SAMHD1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12586-1-AP ( SAMHD1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 THP-1 cells were intracellularly stained with 0.2 µg Anti-Human SAMHD1 (Product # 12586-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), or 0.2 µg Control Antibody (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 K-562 cells were stained with 0.2ug SAMHD1 antibody (12586-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SAMHD1 (IP:12586-1-AP, 3ug; Detection:12586-1-AP 1:300) with K-562 cells lysate 2400ug.