HPA044653

antibody from Atlas Antibodies

Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA044653

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA044653, RRID:AB_2679036

Product name

Anti-SPI1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

EDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWD
FHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRH
MELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYP
SLSPAQPSSDEEEG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human bone marrow and pancreas tissues using Anti-SPI1 antibody. Corresponding SPI1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows strong positivity in a subset of cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN