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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AP32911PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti SPI1 / PU.1 (20-70)
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acid 20-70 of Human PU.1
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Feline, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of PU.1 in U937 cell lysate in the 1) absence and 2) presence of immunizing peptide using SPI1 / PU.1 Antibody Cat.-No AP32911PU-N at 4 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 µg/106 cells. Green peak represents isotype control: Red represents anti-PU.1 antibody.
