R33547

antibody from NSJ Bioreagents

Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R33547

Provider

NSJ Bioreagents

Product name

PU.1 Antibody

Antibody type

Polyclonal

Antigen

Amino acids DLYQRQTHEYY were used as the immunogen for this PU.1 antibody.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Aliquot and store the PU.1 antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Daudi lysate with PU.1 antibody at 0.03ug/ml. Predicted molecular weight ~31kDa but routinely observed at ~40kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human spleen tissue with PU.1 antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon (lymphoid aggregate) tissue with PU.1 antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.