H00006688-M02

antibody from Abnova Corporation

Targeting: SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006688-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006688-M02, RRID:AB_913827

Product name

SPI1 monoclonal antibody (M02), clone 2G1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SPI1.

Antigen sequence

MEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSS
DGESHSDHYWDFHPHHVHSEFESFAENNFTELQSV
QPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQV
SYLPRMCLQYPSLSP


Isotype

IgG

Antibody clone number

2G1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SPI1 monoclonal antibody (M02), clone 2G1. Western Blot analysis of SPI1 expression in Raw 264.7.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SPI1 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SPI1 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol