PA5-56557
antibody from Invitrogen Antibodies
Targeting: MRTFA
BSAC, KIAA1438, MAL, MKL, MKL1, MRTF-A
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-56557 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MKL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EQEKRAQQPA PAPAPLGTPV KQENSFSSCQ LSQQPLGPAH PFNPSLAAPA TNHIDPCAVA PGPPSVVVKQ EALQPEPEPV PAPQLLLGPQ GPSLIKGVAP PTLITDSTGT HLVLTVTNKN ADSPG
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MKL1 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a MKL1 Polyclonal Antibody (Product # PA5-56557). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MKL1 Polyclonal Antibody (Product # PA5-56557) and a ~145 kDa band was observed across the panel tested. An uncharacterized band (*) at ~ 80 and 45 kDa was also observed in HEK-293. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HEK-293 (Lane 2) and DU 145 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MKL1 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a MKL1 Polyclonal Antibody (Product # PA5-56557).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MKL1 in human lymph node using a MKL1 Polyclonal Antibody (Product # PA5-56557) shows cytoplasmic positivity in lymphocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MKL1 in human lymph node using MKL1 Polyclonal Antibody (Product # PA5-56557) shows moderate positivity in lymphoid cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MKL1 in human lung using MKL1 Polyclonal Antibody (Product # PA5-56557) shows moderate cytoplasmic positivity in macrophages.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MKL1 in human liver using MKL1 Polyclonal Antibody (Product # PA5-56557) shows very weak positivity in hepatocytes.