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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Flow cytometry [2]
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- Product number
- TA504254 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504254, RRID:AB_11126436
- Product name
- FXN (Frataxin) mouse monoclonal antibody, clone OTI1C12 (formerly 1C12)
- Antibody type
- Monoclonal
- Description
- FXN (Frataxin) mouse monoclonal antibody, clone OTI1C12 (formerly 1C12)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FXN
- Isotype
- IgG
- Antibody clone number
- OTI1C12
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FXN (RC204880, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FXN.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FXN mouse monoclonal antibody (TA504254) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FXN(RC204880).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504254)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504254)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504254)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504254)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504254)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504254)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC204880 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FXN antibody(TA504254), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-FXN antibody(TA504254),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
