PA5-20782

antibody from Invitrogen Antibodies

Targeting: STING1 ERIS, FLJ38577, MITA, MPYS, NET23, STING, TMEM173

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20782

Provider

Invitrogen Antibodies

Product name

STING Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is A-20 cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of A-20 cell lysate using a MPYS polyclonal antibody (Product # PA5-20782) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: STING Polyclonal Antibody (Product # PA5-20782) (1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: STING Polyclonal Antibody (Product # PA5-20782) (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: STING Polyclonal Antibody (Product # PA5-20782) (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A20 cells using a MPYS polyclonal antibody (Product # PA5-20782) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of A20 cells using STING Polyclonal Antibody (Product # PA5-20782) at 5 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with STING Polyclonal Antibody (Product # PA5-20782) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry staining of A20 cells using a MPYS polyclonal antibody (Product # PA5-20782) at a 5 µg/mL dilution.