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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34765 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LPIN1 Recombinant Rabbit Monoclonal Antibody (JG37-69)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562, SH-SY-5Y, HUVEC, PC-3M, human liver cancer tissue, human colon tissue, human skeletal muscle tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG37-69
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lipin 1 in Positive control: Line 1: K562 Line 2: SH-SY-5Y Line 3: Mouse lung. Samples were incubated with Lipin 1 monoclonal antibody (Product # MA5-34765), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Lipin 1 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Lipin 1 monoclonal antibody (Product # MA5-34765), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Lipin 1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Lipin 1 monoclonal antibody (Product # MA5-34765), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Lipin 1 in paraffin-embedded human skeletal muscle tissue. Samples were incubated with Lipin 1 monoclonal antibody (Product # MA5-34765), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Lipin 1 in paraffin-embedded human liver cancer tissue. Samples were incubated with Lipin 1 monoclonal antibody (Product # MA5-34765), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Lipin 1 in paraffin-embedded human colon tissue. Samples were incubated with Lipin 1 monoclonal antibody (Product # MA5-34765), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Lipin 1 in PC-3M cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Lipin 1 monoclonal antibody (Product # MA5-34765) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
