NBP1-90588

antibody from Novus Biologicals

Targeting: ESPN DFNB36

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-90588

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-90588, RRID:AB_11015490

Product name

Rabbit Polyclonal espin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human espin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Morphology of GNAT3-immunoreactive chemosensory cells in the rat larynx.

Masuda H, Nakamuta N, Yamamoto Y
Journal of anatomy 2019 Feb;234(2):149-164

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Differential effects of depot formulations of GnRH agonist leuprorelin and antagonist degarelix on the seminiferous epithelium of the rat testis.

Hori JI, Koga D, Kakizaki H, Watanabe T
Biomedical research (Tokyo, Japan) 2018;39(4):197-214

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: espin Antibody [NBP1-90588] - Simple Western lane view shows a specific band for ESPN in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: espin Antibody [NBP1-90588] - Electropherogram image(s) of corresponding Simple Western lane view. espin antibody was used at 1:20 dilution on RT-4 lysate(s).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: espin Antibody [NBP1-90588] - Analysis in human cell line SK-BR-3.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: espin Antibody [NBP1-90588] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human testis shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining in human testis and skeletal muscle tissues using NBP1-90588 antibody. Corresponding ESPN RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human kidney shows strong positivity in luminal membrane in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.