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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- MA5-24300 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLF2 Monoclonal Antibody (665333)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross-reactivity with rhKLF1, 4, 5, 6, 10, 12, 17, recombinant mouse KLF4 or 15 is observed.
- Antibody clone number
- 665333
- Concentration
- 0.5 mg/mL
Submitted references IRF2BP2 prevents ox-LDL-induced inflammation and EMT in endothelial cells via regulation of KLF2.
Jiang Y, Shen Q
Experimental and therapeutic medicine 2021 May;21(5):481
Experimental and therapeutic medicine 2021 May;21(5):481
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of KLF2 was detected in immersion fixed BG01V human embryonic stem cells using human KLF2 Monoclonal Antibody (Product # MA5-24300) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of BG01V human embryonic stem cells were stained with mouse Anti-human KLF2 Monoclonal Antibody (Product # MA5-24300) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 IRF2BP2 increases the expression of KLF2 and improves endothelial function. (A) The expression levels of ICAM-1 and VCAM-1 were determined using reverse transcription-quantitative PCR analysis. (B) The protein expression levels of KLF2, p-eNOS, total eNOS and GAPDH were measured using western blot analysis. (C) A Nitrate/Nitrite assay kit was utilized to estimate the release of NO. (D) The expression of KLF2 was evaluated by immunofluorescence analysis (magnification, x200). Scale bar, 50 um. ** P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 IRF2BP2 attenuates inflammation and EMT via KLF2. (A) The interaction between IRF2BP2 and KLF2 was confirmed using a Co-IP assay. (B) HUVECs were transfected with scrambled siRNA, siKLF2#1 or siKLF2#2. The mRNA expression levels of KLF2 were measured using reverse transcription-quantitative PCR analysis. *** P
