Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-75301 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARR3 in Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate. Samples were incubated with ARR3 polyclonal antibody (Product # PA5-75301) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-ARR3 Polyclonal Antibody (Product # PA5-75301) and 50kDa band corresponding to ARR3 was observed only in Mouse Retina and absent in rest of the samples that are reported negative for ARR3 expression. Additional uncharacterized bands were observed in certain cell lines. Whole cell extracts of HEK-293 (Lane 1), RAW 264.7 (Lane 2), PC-12 (Lane 3), Hep G2 (Lane 4), tissue extracts of Mouse Retina (Lane 5), Mouse Eye (Lane 6), Rat Eye (Lane 7), Mouse Brain (Lane 8), Rat Brain (Lane 9) and Mouse Skeletal Muscle (Lane 10) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:500 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ARR3 in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with ARR3 polyclonal antibody (Product # PA5-75301) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.