PA5-96658

antibody from Invitrogen Antibodies

Targeting: CHRAC1 CHRAC15, YCL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96658

Provider

Invitrogen Antibodies

Product name

CHRAC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MADVVVGKDK GGEQRLISLP LSRIRVIMKS SPEVSSINQE ALVLTAKATE LFVQCLATYS YRHGSGKEKK VLTYSDLANT AQQSETFQFL ADILPKKILA SKYLKMLKEE KREEDEENDN DNESDHDEAD S; Positive Samples: U-87MG, A549, HepG2, 293T, BxPC-3; Cellular Location: Nucleus

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CHRAC1 Polyclonal antibody (Product # PA5-96658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CHRAC1 in extracts of various cell lines using CHRAC1 Polyclonal Antibody (Product # PA5-96658) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CHRAC1 in extracts of various cell lines using CHRAC1 Polyclonal Antibody (Product # PA5-96658) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CHRAC1 in paraffin-embedded mouse brain using CHRAC1 Polyclonal Antibody (Product # PA5-96658) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CHRAC1 in paraffin-embedded human stomach using CHRAC1 Polyclonal Antibody (Product # PA5-96658) at a dilution of 1:100.