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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- UM800019 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WFDC2 Monoclonal Antibody (UMAB88), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB88
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HT-29 cell lysate (35µg) by using anti-WFDC2 monoclonal antibody (Clone UMAB88
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Dilution: 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded 3 human ovarian cancer using anti-HE4 clone UMAB88 mouse monoclonal antibody at 1:200 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded 3 human endometrial cancer using anti-HE4 clone UMAB88 mouse monoclonal antibody at 1:200 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung cancer using anti-HE4 clone UMAB88 mouse monoclonal antibody at 1:200 dilution of mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110°C for 3 min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrium tissue using anti-WFDC2 mouse monoclonal antibody. (UM800019; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WFDC2 mouse monoclonal antibody (UM800019). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
