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- References [26]
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- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 11576-2-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11576-2-AP, RRID:AB_2156943
- Product name
- OGT antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated OGT antibody (Cat. #11576-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references DNA Polymerase Iota Promotes Esophageal Squamous Cell Carcinoma Proliferation Through Erk-OGT-Induced G6PD Overactivation.
OGT regulated O-GlcNacylation promotes migration and invasion by activating IL-6/STAT3 signaling in NSCLC cells.
O‑GlcNAcylation contributes to intermittent hypoxia‑associated vascular dysfunction via modulation of MAPKs but not CaMKII pathways.
Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.
O-GlcNAc modification regulates MTA1 transcriptional activity during breast cancer cell genotoxic adaptation.
Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
GFPT2 promotes metastasis and forms a positive feedback loop with p65 in colorectal cancer.
Proteomic profiling and genome-wide mapping of O-GlcNAc chromatin-associated proteins reveal an O-GlcNAc-regulated genotoxic stress response.
Long non-coding RNA LINC00707 acts as a competing endogenous RNA to enhance cell proliferation in colorectal cancer.
Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.
O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion.
MicroRNA-24 inhibits the oxidative stress induced by vascular injury by activating the Nrf2/Ho-1 signaling pathway.
Pharmacological inhibition of dihydroorotate dehydrogenase induces apoptosis and differentiation in acute myeloid leukemia cells.
O-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response.
High O-linked N-acetylglucosamine transferase expression predicts poor survival in patients with early stage lung adenocarcinoma.
GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.
Identification and characterization of a missense mutation in the O-linked β-N-acetylglucosamine (O-GlcNAc) transferase gene that segregates with X-linked intellectual disability.
Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase.
O-GlcNAc transferase promotes fatty liver-associated liver cancer through inducing palmitic acid and activating endoplasmic reticulum stress.
Downregulation of O-linked N-acetylglucosamine transferase by RNA interference decreases MMP9 expression in human esophageal cancer cells.
Differential membranous E-cadherin expression, cell proliferation and O-GlcNAcylation between primary and metastatic nodal lesion in colorectal cancer.
Glucose-insulin-potassium solution protects ventricular myocytes of neonatal rat in an in vitro coverslip ischemia/reperfusion model.
Alterations in left ventricular function during intermittent hypoxia: Possible involvement of O-GlcNAc protein and MAPK signaling.
O-GlcNAcylation plays a role in tumor recurrence of hepatocellular carcinoma following liver transplantation.
O-linked N-acetylglucosamine transferase (OGT) is overexpressed and promotes O-linked protein glycosylation in esophageal squamous cell carcinoma.
A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay.
Su Z, Gao A, Li X, Zou S, He C, Wu J, Ding WQ, Zhou J
Frontiers in oncology 2021;11:706337
Frontiers in oncology 2021;11:706337
OGT regulated O-GlcNacylation promotes migration and invasion by activating IL-6/STAT3 signaling in NSCLC cells.
Ge X, Peng X, Li M, Ji F, Chen J, Zhang D
Pathology, research and practice 2021 Sep;225:153580
Pathology, research and practice 2021 Sep;225:153580
O‑GlcNAcylation contributes to intermittent hypoxia‑associated vascular dysfunction via modulation of MAPKs but not CaMKII pathways.
Guo X, Deng Y, Zhan L, Shang J, Liu H
Molecular medicine reports 2021 Nov;24(5)
Molecular medicine reports 2021 Nov;24(5)
Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.
Ou W, Liang Y, Qin Y, Wu W, Xie M, Zhang Y, Zhang Y, Ji L, Yu H, Li T
Redox biology 2021 Jul;43:101994
Redox biology 2021 Jul;43:101994
O-GlcNAc modification regulates MTA1 transcriptional activity during breast cancer cell genotoxic adaptation.
Xie X, Wu Q, Zhang K, Liu Y, Zhang N, Chen Q, Wang L, Li W, Zhang J, Liu Y
Biochimica et biophysica acta. General subjects 2021 Aug;1865(8):129930
Biochimica et biophysica acta. General subjects 2021 Aug;1865(8):129930
Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
Wu F, Shao Q, Cheng Z, Xiong X, Fang K, Zhao Y, Dong R, Xu L, Lu F, Chen G
Chinese medicine 2021 Aug 16;16(1):78
Chinese medicine 2021 Aug 16;16(1):78
GFPT2 promotes metastasis and forms a positive feedback loop with p65 in colorectal cancer.
Liu L, Pan Y, Ren X, Zeng Z, Sun J, Zhou K, Liang Y, Wang F, Yan Y, Liao W, Ding Y, Liu X, Liang L
American journal of cancer research 2020;10(8):2510-2522
American journal of cancer research 2020;10(8):2510-2522
Proteomic profiling and genome-wide mapping of O-GlcNAc chromatin-associated proteins reveal an O-GlcNAc-regulated genotoxic stress response.
Liu Y, Chen Q, Zhang N, Zhang K, Dou T, Cao Y, Liu Y, Li K, Hao X, Xie X, Li W, Ren Y, Zhang J
Nature communications 2020 Nov 19;11(1):5898
Nature communications 2020 Nov 19;11(1):5898
Long non-coding RNA LINC00707 acts as a competing endogenous RNA to enhance cell proliferation in colorectal cancer.
Wang H, Luan H, Zhan T, Liu X, Song J, Dai H
Experimental and therapeutic medicine 2020 Feb;19(2):1439-1447
Experimental and therapeutic medicine 2020 Feb;19(2):1439-1447
Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.
Marmor-Kollet H, Siany A, Kedersha N, Knafo N, Rivkin N, Danino YM, Moens TG, Olender T, Sheban D, Cohen N, Dadosh T, Addadi Y, Ravid R, Eitan C, Toth Cohen B, Hofmann S, Riggs CL, Advani VM, Higginbottom A, Cooper-Knock J, Hanna JH, Merbl Y, Van Den Bosch L, Anderson P, Ivanov P, Geiger T, Hornstein E
Molecular cell 2020 Dec 3;80(5):876-891.e6
Molecular cell 2020 Dec 3;80(5):876-891.e6
O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion.
Han X, Li X, Liu H, Zhang H, Li A, Dong M, Xu Y, Yan B, Sui L, Kong Y
Oncology reports 2019 Nov;42(5):2065-2074
Oncology reports 2019 Nov;42(5):2065-2074
MicroRNA-24 inhibits the oxidative stress induced by vascular injury by activating the Nrf2/Ho-1 signaling pathway.
Zhang J, Cai W, Fan Z, Yang C, Wang W, Xiong M, Ma C, Yang J
Atherosclerosis 2019 Nov;290:9-18
Atherosclerosis 2019 Nov;290:9-18
Pharmacological inhibition of dihydroorotate dehydrogenase induces apoptosis and differentiation in acute myeloid leukemia cells.
Wu D, Wang W, Chen W, Lian F, Lang L, Huang Y, Xu Y, Zhang N, Chen Y, Liu M, Nussinov R, Cheng F, Lu W, Huang J
Haematologica 2018 Sep;103(9):1472-1483
Haematologica 2018 Sep;103(9):1472-1483
O-GlcNAc modification of eIF4GI acts as a translational switch in heat shock response.
Zhang X, Shu XE, Qian SB
Nature chemical biology 2018 Oct;14(10):909-916
Nature chemical biology 2018 Oct;14(10):909-916
High O-linked N-acetylglucosamine transferase expression predicts poor survival in patients with early stage lung adenocarcinoma.
Lin YC, Lin CH, Yeh YC, Ho HL, Wu YC, Chen MY, Chou TY
Oncotarget 2018 Jul 24;9(57):31032-31044
Oncotarget 2018 Jul 24;9(57):31032-31044
GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.
Zhang X, Wang L, Lak B, Li J, Jokitalo E, Wang Y
Developmental cell 2018 Apr 23;45(2):245-261.e6
Developmental cell 2018 Apr 23;45(2):245-261.e6
Identification and characterization of a missense mutation in the O-linked β-N-acetylglucosamine (O-GlcNAc) transferase gene that segregates with X-linked intellectual disability.
Vaidyanathan K, Niranjan T, Selvan N, Teo CF, May M, Patel S, Weatherly B, Skinner C, Opitz J, Carey J, Viskochil D, Gecz J, Shaw M, Peng Y, Alexov E, Wang T, Schwartz C, Wells L
The Journal of biological chemistry 2017 May 26;292(21):8948-8963
The Journal of biological chemistry 2017 May 26;292(21):8948-8963
Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase.
Hu CW, Worth M, Fan D, Li B, Li H, Lu L, Zhong X, Lin Z, Wei L, Ge Y, Li L, Jiang J
Nature chemical biology 2017 Dec;13(12):1267-1273
Nature chemical biology 2017 Dec;13(12):1267-1273
O-GlcNAc transferase promotes fatty liver-associated liver cancer through inducing palmitic acid and activating endoplasmic reticulum stress.
Xu W, Zhang X, Wu JL, Fu L, Liu K, Liu D, Chen GG, Lai PB, Wong N, Yu J
Journal of hepatology 2017 Aug;67(2):310-320
Journal of hepatology 2017 Aug;67(2):310-320
Downregulation of O-linked N-acetylglucosamine transferase by RNA interference decreases MMP9 expression in human esophageal cancer cells.
Qiao Z, Dang C, Zhou B, Li S, Zhang W, Jiang J, Zhang J, Ma Y, Kong R, Ma Z
Oncology letters 2016 May;11(5):3317-3323
Oncology letters 2016 May;11(5):3317-3323
Differential membranous E-cadherin expression, cell proliferation and O-GlcNAcylation between primary and metastatic nodal lesion in colorectal cancer.
Jang TJ
Pathology, research and practice 2016 Feb;212(2):113-9
Pathology, research and practice 2016 Feb;212(2):113-9
Glucose-insulin-potassium solution protects ventricular myocytes of neonatal rat in an in vitro coverslip ischemia/reperfusion model.
Chun WJ, Nah DY, Bae JH, Chung JW, Lee H, Moon IS
Korean circulation journal 2015 May;45(3):234-41
Korean circulation journal 2015 May;45(3):234-41
Alterations in left ventricular function during intermittent hypoxia: Possible involvement of O-GlcNAc protein and MAPK signaling.
Guo X, Shang J, Deng Y, Yuan X, Zhu D, Liu H
International journal of molecular medicine 2015 Jul;36(1):150-8
International journal of molecular medicine 2015 Jul;36(1):150-8
O-GlcNAcylation plays a role in tumor recurrence of hepatocellular carcinoma following liver transplantation.
Zhu Q, Zhou L, Yang Z, Lai M, Xie H, Wu L, Xing C, Zhang F, Zheng S
Medical oncology (Northwood, London, England) 2012 Jun;29(2):985-93
Medical oncology (Northwood, London, England) 2012 Jun;29(2):985-93
O-linked N-acetylglucosamine transferase (OGT) is overexpressed and promotes O-linked protein glycosylation in esophageal squamous cell carcinoma.
Qiao Z, Dang C, Zhou B, Li S, Zhang W, Jiang J, Zhang J, Kong R, Ma Y
Journal of biomedical research 2012 Jul;26(4):268-73
Journal of biomedical research 2012 Jul;26(4):268-73
A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay.
Zhang L, Ren F, Li J, Ma X, Wang P
Biological procedures online 2009 Dec 3;11:170-83
Biological procedures online 2009 Dec 3;11:170-83
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 11576-2-AP(OGT antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-OGT (IP:11576-2-AP, 3ug; Detection:11576-2-AP 1:1000) with mouse brain tissue lysate 8000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The OGT antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human OGT. This antibody recognizes human,mouse,rat antigen. The OGT antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
