GTX109939

antibody from GeneTex

Targeting: OGT FLJ23071, HRNT1, MGC22921, O-GLCNAC, OGT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX109939

Provider

GeneTex

Proper citation

GeneTex Cat#GTX109939, RRID:AB_1951060

Product name

O-GlcNAc transferase antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Tissue Engineering and Dental Implantology: Biomaterials, New Technologies, and Stem Cells.

Sammartino G, Dohan Ehrenfest DM, Shibli JA, Galindo-Moreno P
BioMed research international 2016;2016:5713168

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Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.

Nagai-Okatani C, Minamino N
PloS one 2016;11(6):e0150210

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Hexosamine-Induced TGF-β Signaling and Osteogenic Differentiation of Dental Pulp Stem Cells Are Dependent on N-Acetylglucosaminyltransferase V.

Chen YJ, Yao CC, Huang CH, Chang HH, Young TH
BioMed research international 2015;2015:924397

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Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin.

Ise H, Goto M, Komura K, Akaike T
Glycobiology 2012 Jun;22(6):788-805

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Dynamic behaviors of vimentin induced by interaction with GlcNAc molecules.

Komura K, Ise H, Akaike T
Glycobiology 2012 Dec;22(12):1741-59

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects OGT protein by Western blot analysis.A. 30 µg PC-12 whole cell lysate/extract7.5 % SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate)A: HeLa7.5% SDS PAGEGTX109939 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX109939 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g A431 whole cell extract C. 30 ?g HeLa whole cell extract D. 30 ?g HepG2 whole cell extract7.5 % SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 50 ?g mouse brain extract5 % SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 30 ?g PC-12 whole cell extract5% SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.A. 30 ?g 293T whole cell extract B. 30 ?g A431 whole cell extract C. 30 ?g HeLa whole cell extract D. 30 ?g HepG2 whole cell extract5% SDS-PAGEO-GlcNAc transferase antibody (GTX109939) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 7.5% SDS-PAGE, and blotted with O-GlcNAc transferase antibody (GTX109939) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: O-GlcNAc transferase protein stained by O-GlcNAc transferase antibody (GTX109939) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of O-GlcNAc transferase protein from A431 whole cell extracts using 5 £gg of O-GlcNAc transferase antibody (GTX109939).Western blot analysis was performed using O-GlcNAc transferase antibody (GTX109939).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

O-GlcNAc transferase antibody detects O-GlcNAc transferase protein at cytoplasm and nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. O-GlcNAc transferase antibody (GTX109939) diluted at 1:500.