838004

antibody from BioLegend

Targeting: OGT FLJ23071, HRNT1, MGC22921, O-GLCNAC, OGT1

Western blot (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

838004

Provider

BioLegend

Proper citation

BioLegend Cat#838004, RRID:AB_2629520

Product name

Purified anti-O-GlcNAc

Antibody type

Monoclonal

Antigen

The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

CTD110.6

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Immunofluorescence staining of CTD110.6 on methanol fixed HeLa cells at 10 µg/ml. Following fixation, the cells were incubated with the primary antibody for one hour at room temperature, followed by incubation with goat anti-mouse IgM Alexa Fluor® 488 secondary antibody (green). The cells were counterstained with DAPI (blue).

Conjugate

Unconjugated