PAB22483

antibody from Abnova Corporation

Targeting: OGT FLJ23071, HRNT1, MGC22921, O-GLCNAC, OGT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22483

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22483, RRID:AB_10965187

Product name

OGT polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant OGT.

Antigen sequence

NMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAF
LDSLPDVKIVKMKCPDGGDNADSSNTALNMPVIPM
NTIAEAVIEMINRGQIQITINGFSISNGLATT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with OGT polyclonal antibody (Cat # PAB22483) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with OGT polyclonal antibody (Cat # PAB22483) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with OGT polyclonal antibody (Cat # PAB22483) shows moderate cytoplasmic positivity in cells in tubules at 1:10-1:20 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)