MA5-24514

antibody from Invitrogen Antibodies

Targeting: RBP7 CRBPIV

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24514

Provider

Invitrogen Antibodies

Product name

RBP7 Monoclonal Antibody (4F4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Peptide Sequence: KLLKPQKVIE QNGDSFTIHT NSSLRNYFVK FKVGEEFDED NRGLDNRKCK SLVIWDNDRL TCIQKGEKKN RGWTHWIEGD KLHLEMFCEG QVCKQTFQRA

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4F4

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin.

Li Q, Zhu Z, Wang L, Lin Y, Fang H, Lei J, Cao T, Wang G, Dang E
Theranostics 2021;11(13):6461-6476

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBP7 / Retinol-Binding Protein 7 in transfected 293T cell line. Lane 1: RBP7 transfected lysate (Predicted MW: 15.5 KDa). Lane 2: Non-transfected lysate. Samples were probed using a Mouse Monoclonal RBP7 / Retinol-Binding Protein 7 (4F4) Antibody (Product # MA5-24514).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RBP7 / Retinol-Binding Protein 7 in transfected 293T cell line. Lane 1: RBP7 transfected lysate (Predicted MW: 15.5 KDa). Lane 2: Non-transfected lysate. Samples were probed using a Mouse Monoclonal RBP7 / Retinol-Binding Protein 7 (4F4) Antibody (Product # MA5-24514).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RBP7 / Retinol-Binding Protein 7 in HeLa cell lysates. Samples were probed using a Mouse Monoclonal RBP7 / Retinol-Binding Protein 7 (4F4) Antibody (Product # MA5-24514) at 10 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RBP7 / Retinol-Binding Protein 7 in HeLa cell lysates. Samples were probed using a Mouse Monoclonal RBP7 / Retinol-Binding Protein 7 (4F4) Antibody (Product # MA5-24514) at 10 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis using recombinant GST tagged RBP7 / Retinol-Binding Protein 7 Antibody (Product # MA5-24514) at 1 ng/mL as the capture antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Heterogeneous expressions and functions of dermal ECs along the arteriovenous axis. ( A ) Zonational expression of representative genes across dermal vascular bed. ECs are rearranged in an arteriovenous order. y axis represents counts. ( B-E ) Immunofluorescence of IGFBP3, RBP7, SELE, MT2A and CD31. Images of CD31 + ECs in the superficial, intermediate, and deep plexus are zoomed in. The image is representative of 11 biological replicates (limbs, n = 4; trunk, n = 4; foreskin, n = 3). The white dotted line marks the interface between epidermis and dermis. Scale bars represent 20 um. ( F ) Single-cell trajectory of dermal EC subtypes by pseudotime analysis. The white line traces the trajectory. ECs in the left panel are colored by clusters, and white numbers in black circles mark the branch nodes of the pseudotime trajectory. ECs in the right panel plot are colored by pseudotime. Color scale: yellow, 'leaf' of the trajectory; purple, 'root' of the trajectory. ( G ) GO pathway enrichment analysis of the marker genes of dermal EC clusters. The color represents -log p value, and the size indicates the rich factor. Cluster A: arteriole ECs; Cluster C1, C2: capillary ECs; Cluster P: post-capillary ECs; Cluster V: venule ECs. ECs: vascular endothelial cells; GO: gene ontology; UMAP: uniform manifold approximation and projection.