686603

antibody from BioLegend

Targeting: CHEK2 bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53

Immunocytochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

686603

Provider

BioLegend

Proper citation

BioLegend Cat#686603, RRID:AB_2617020

Product name

Alexa Fluor® 594 anti-Chk2

Antibody type

Monoclonal

Antigen

Purified recombinant fragment of Chk2 (AA: 481-531) expressed in E.Coli.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

P81C12B8

Vial size

25 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. The cells were intracellularly stained with 0.5 µg/ml Alexa Fluor® 594 anti-Chk2 antibody (clone P81C12B8) overnight at 4°C. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.

Conjugate

Yellow dye