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antibody from Invitrogen Antibodies
Targeting: CHEK2
bA444G7, CDS1, CHK2, HuCds1, PP1425, RAD53
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-87441 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MSRESDVEAQ QSHGSSACSQ PHGSVTQSQG SSSQSQGISS SSTSTMPNSS QSSHSSSGTL SSLETVSTQE LYSIPEDQEP EDQEPEEPTP APWARLWALQ DGFANLECVN DNYWFGRDKS CEYCFDEPLL KRTDKYRTYS KKHFRIFREV GPKNSYIAYI EDHSGNGTFV NTELVGKGKR RPLNNNSEIA LSLSRNKVFV FFDLTVDDQS VYPKALRDEY; Positive Samples: Raji, MCF7, K-562, Jurkat; Cellular Location: Nucleus, Nucleus, PML body, nucleoplasm
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.71 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using CHEK2 Polyclonal antibody (Product # PA5-87441) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CHK2 in extracts of various cell lines using CHK2 Polyclonal Antibody (Product # PA5-87441) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHK2 was performed in HeLa cells using CHK2 Polyclonal Antibody (Product # PA5-87441).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CHK2 was performed in A549 cells using CHK2 Polyclonal Antibody (Product # PA5-87441).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHK2 in paraffin-embedded human lung cancer using CHK2 Polyclonal Antibody (Product # PA5-87441) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of CHK2 was performed in 200 µg extracts of MCF-7 cells using CHK2 Polyclonal Antibody (Product # PA5-87441). Western blot was performed from the immunoprecipitate using CHK2 Polyclonal Antibody.
