Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
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Validation data
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- Product number
- TA319180 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Nag-1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Nag-1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GDF15
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- In this ELISA, NAG-1 was captured from human serum using the following antibodies): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 ug/mL. After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C.
- Validation comment
- ELISA