NB600-1469

antibody from Novus Biologicals

Targeting: HSPA1A HSP70-1, HSPA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Gel shift (Data presented on provider website)

Antibody Data

Product number

NB600-1469

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-1469, RRID:AB_2119820

Product name

Mouse Monoclonal HSP70/HSPA1A Antibody

Antibody type

Monoclonal

Description

Unpurified. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Drosophila, Porcine, Simian, Yeast

Host

Mouse

Isotype

IgG

Vial size

50uL

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Acidic microenvironment plays a key role in human melanoma progression through a sustained exosome mediated transfer of clinically relevant metastatic molecules.

Boussadia Z, Lamberti J, Mattei F, Pizzi E, Puglisi R, Zanetti C, Pasquini L, Fratini F, Fantozzi L, Felicetti F, Fecchi K, Raggi C, Sanchez M, D'Atri S, Carè A, Sargiacomo M, Parolini I
Journal of experimental & clinical cancer research : CR 2018 Oct 5;37(1):245

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Blocking Hsp70 enhances the efficiency of amphotericin B treatment against resistant Aspergillus terreus strains.

Blatzer M, Blum G, Jukic E, Posch W, Gruber P, Nagl M, Binder U, Maurer E, Sarg B, Lindner H, Lass-Flörl C, Wilflingseder D
Antimicrobial agents and chemotherapy 2015 Jul;59(7):3778-88

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a Hsp70 monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa, isolated from various coves in Rhode Island (indicated above the lanes).

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Immunoprecipitation of Heat Shock Protein 70 (Hsp70) was performed on HeLa cells. Antigen:antibody complexes were formed by incubating 500ug whole cell lysate with 2ug of Hsp70 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (3A3) [NB600-1469] - Both normal and cancer biopsies of deparaffinized human Testis tissue.