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antibody from Invitrogen Antibodies
Targeting: EHMT2
BAT8, C6orf30, Em:AF134726.3, G9A, KMT1C, NG36/G9a
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-34971 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EHMT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 2.63 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EHMT2/G9a using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate (D) 30 µg HepG2 whole cell lysate and E) 30 µg A375 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an EHMT2/G9a polyclonal antibody (Product # PA5-34971) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EHMT2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EHMT2 Polyclonal Antibody (Product # PA5-34971) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EHMT2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EHMT2 Polyclonal Antibody (Product # PA5-34971) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EHMT2/G9a showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min and stained using an EHMT2/G9a polyclonal antibody (Product # PA5-34971) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of EHMT2 was performed in paraffin-embedded human rectal carcinoma tissue using EHMT2 Polyclonal Antibody (Product # PA5-34971) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EHMT2 Polyclonal Antibody detects EHMT2 protein at on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. EHMT2 Polyclonal Antibody (Product # PA5-34971) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of G9a was performed in HeLa whole cell extracts using 5 µg of EHMT2 Polyclonal Antibody (Product # PA5-34971). Samples were transferred to a membrane and probed with EHMT2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
