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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 641001 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#641001, RRID:AB_1227660
- Product name
- Purified anti-Histone H3 Phospho (Ser28)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3.
- Description
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin.Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store at 4° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of extracts from untreated Hela cells (lane 1) or overnight nocodazole-treated Hela cells (lane 2), using anti-phospho-Histone H3 (Ser28), clone HTA28.
- Conjugate
- Unconjugated
