PA5-21382

antibody from Invitrogen Antibodies

Targeting: CKB CKBB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21382

Provider

Invitrogen Antibodies

Product name

Creatine Kinase BB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: H1299, HeLaS3, mouse brain, rat brain.

Concentration

0.54 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382). Sample (30 µg of whole cell lysate). A: H1299. B: HeLa S3. 12% SDS PAGE. Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with Creatine kinase (brain) antibody Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Creatine Kinase BB in paraformaldehyde-fixed HeLa cells using a Creatine Kinase BB polyclonal antibody (Product # PA5-21382) at a 1:100 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB antibody (Product # PA5-21382) at 1:100 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Creatine Kinase BB Polyclonal Antibody detects Creatine kinase (brain) protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain) (Product # PA5-21382) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.