Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP32844PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti GAP43 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.
- Reactivity
- Mouse
- Host
- Chicken/Avian
- Vial size
- 0.2 ml
- Concentration
- 2.0 mg IgY/ml (based on Bradford assay readings using BSA as a standard).
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- GAP43 staining of cultured cortical neurons purified from the neonatal Mouse brain (green satining). Red staining corresponds to a Rabbit anti- Alpha III Spectrin. Blue staining corresponds to the Hoechst nuclear satin.