AF3580

antibody from Novus Biologicals

Targeting: FKBP8 FKBP38, FKBPr38

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3580

Provider

Novus Biologicals

Product name

Goat Polyclonal FKBP38 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human, mouse, and rat FKBP38 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human FKBP-12, -12.6, -13, -25, -51, or -52 is observed.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Hepatitis C virus NS5A activates the mammalian target of rapamycin (mTOR) pathway, contributing to cell survival by disrupting the interaction between FK506-binding protein 38 (FKBP38) and mTOR.

Peng L, Liang D, Tong W, Li J, Yuan Z
The Journal of biological chemistry 2010 Jul 2;285(27):20870-81

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat FKBP38 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA-MB-468 human breast cancer cell line, A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP38 at approximately 60 - 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human FKBP38 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA-MB-468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FKBP38 at approximately 58 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

FKBP38 in Human Intestine. FKBP38 was detected in immersion fixed paraffin-embedded sections of human intestine using 15 µg/mL Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in rat or mouse tissue.