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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB3580 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat FKBP38 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human, mouse and rat FKBP38 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q14318- Isotype
- IgG
- Antibody clone number
- 391505
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Phosphatidic acid activates mammalian target of rapamycin complex 1 (mTORC1) kinase by displacing FK506 binding protein 38 (FKBP38) and exerting an allosteric effect.
Yoon MS, Sun Y, Arauz E, Jiang Y, Chen J
The Journal of biological chemistry 2011 Aug 26;286(34):29568-74
The Journal of biological chemistry 2011 Aug 26;286(34):29568-74
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human/Mouse/Rat FKBP38 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat FKBP38 Monoclonal Antibody (Catalog # MAB3580) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FKBP38 at approximately 60 to 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
