Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 37-6100 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNIP1 Monoclonal Antibody (1A11E3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A11E3
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references TNF receptor-activated factor 2 mediates cardiac protection through noncanonical NF-κB signaling.
The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes.
Hematopoietic progenitor cell lines with myeloid and lymphoid potential.
ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling.
A large scale shRNA barcode screen identifies the circadian clock component ARNTL as putative regulator of the p53 tumor suppressor pathway.
Evans S, Tzeng HP, Veis DJ, Matkovich S, Weinheimer C, Kovacs A, Barger PM, Mann DL
JCI insight 2018 Feb 8;3(3)
JCI insight 2018 Feb 8;3(3)
The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes.
Afonina IS, Van Nuffel E, Baudelet G, Driege Y, Kreike M, Staal J, Beyaert R
EMBO reports 2016 Jun;17(6):914-27
EMBO reports 2016 Jun;17(6):914-27
Hematopoietic progenitor cell lines with myeloid and lymphoid potential.
Redecke V, Wu R, Zhou J, Finkelstein D, Chaturvedi V, High AA, Häcker H
Nature methods 2013 Aug;10(8):795-803
Nature methods 2013 Aug;10(8):795-803
ABIN1 protein cooperates with TAX1BP1 and A20 proteins to inhibit antiviral signaling.
Gao L, Coope H, Grant S, Ma A, Ley SC, Harhaj EW
The Journal of biological chemistry 2011 Oct 21;286(42):36592-602
The Journal of biological chemistry 2011 Oct 21;286(42):36592-602
A large scale shRNA barcode screen identifies the circadian clock component ARNTL as putative regulator of the p53 tumor suppressor pathway.
Mullenders J, Fabius AW, Madiredjo M, Bernards R, Beijersbergen RL
PloS one 2009;4(3):e4798
PloS one 2009;4(3):e4798
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HEK293 cell lysates using Ms anti-ABIN-1 (Product # 37-6100).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ABIN-1 was performed using whole cell extracts (30 µg lysate) of A-431 (Lane 1), PC-3 (Lane 2), HEL 92.1.7 (Lane 3) and K-562 (Lane 4). The blots were probed with Anti-ABIN-1 Mouse monoclonal Antibody (Product # 37-6100, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 72 kDa band corresponding to ABIN-1 was observed across the cell lines tested. A lower band of 48 kDa was observed showing NEMO/IKK gamma which binds in complex. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
