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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- GTX628143 - Provider product page
- Provider
- GeneTex
- Product name
- Citrate synthetase antibody [GT1761]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.A. 30 ?g zebrafish brain lysate/extract10% SDS-PAGECitrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extractE. 30 ?g A375 whole cell lysate/extract12% SDS-PAGECitrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.A. 50 ?g mouse brain lysate/extract12% SDS-PAGECitrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.A. 30 ?g zebrafish heart lysate/extract10% SDS-PAGECitrate synthetase antibody [GT1761] (GTX628143) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] (GTX628143) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody detects CS protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. Citrate synthetase antibody (GTX628143) dilution: 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Citrate synthetase antibody [GT1761] (GTX628143) dilution: 1:200.
