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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-61614 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61614, RRID:AB_2290670
- Product name
- Rabbit Polyclonal CAD Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Phospho-ΔNp63α/SREBF1 protein interactions: bridging cell metabolism and cisplatin chemoresistance.
Identification of CAD as an androgen receptor interactant and an early marker of prostate tumor recurrence.
Huang Y, Bell LN, Okamura J, Kim MS, Mohney RP, Guerrero-Preston R, Ratovitski EA
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
Cell cycle (Georgetown, Tex.) 2012 Oct 15;11(20):3810-27
Identification of CAD as an androgen receptor interactant and an early marker of prostate tumor recurrence.
Morin A, Fritsch L, Mathieu JR, Gilbert C, Guarmit B, Firlej V, Gallou-Kabani C, Vieillefond A, Delongchamps NB, Cabon F
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Jan;26(1):460-7
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Jan;26(1):460-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CAD Antibody [NB100-61614] - Detection of Human and Mouse CAD by Western Blot. Samples: Whole cell lysate from He L a (5, 15 and 50 ug), 293T (50 ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CAD Antibody [NB100-61614] - Detection of Human and Mouse CAD on HeLa whole cell lysate using NB100-61614. CAD was also immunoprecipitated by rabbit anti-CAD antibody NB100-61612.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CAD Antibody [NB100-61614] - Whole cell lysate (50 ug) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CAD Antibody [NB100-61614] - Detection of human CAD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAD antibody NB100-61614 (lot NB100-61614-3) used for IP at 6 ug per reaction. CAD was also immunoprecipitated by a previous lot of this antibody (lot NB100-61614-2) and another rabbit anti-CAD antibody. For blotting immunoprecipitated CAD, NB100-61614 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:200 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
