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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27765 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TPL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 0.98 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COT/MAP3K8 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a COT/MAP3K8 polyclonal antibody (Product # PA5-27765) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TPL2 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TPL2 Polyclonal Antibody (Product # PA5-27765) at a dilution of 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TPL2 Polyclonal Antibody (Product # PA5-27765). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TPL2 Polyclonal Antibody (Product # PA5-27765) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TPL2 Polyclonal Antibody (Product # PA5-27765). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TPL2 Polyclonal Antibody (Product # PA5-27765) diluted at 1:500,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COT/MAP3K8 in methanol-fixed HeLa cells using a COT/MAP3K8 polyclonal antibody (Product # PA5-27765) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using MEKK8 (COT) (Product # PA5-27765) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TPL2 Polyclonal Antibody detects MAP3K8 protein at cytoplasm on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. TPL2 Polyclonal Antibody (Product # PA5-27765) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
