Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21468 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, C2C12, PC-12, Rat2.
- Concentration
- 0.59 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of CSE1L using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a CSE1L polyclonal antibody (Product # PA5-21468) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CSE1L using A) 30 µg PC-12 whole cell lysate and B) 30 µg Rat2 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a CSE1L polyclonal antibody (Product # PA5-21468) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CSE1L using 30 µg of A) 293T (B) A431 (C) H1299 and D) HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CSE1L polyclonal antibody (Product # PA5-21468) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CAS was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CAS Polyclonal Antibody (Product # PA5-21468) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CAS Polyclonal Antibody (Product # PA5-21468). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CAS Polyclonal Antibody (Product # PA5-21468) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CAS was performed by separating 30 µg of various whole cell lysates by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CAS Polyclonal Antibody (Product # PA5-21468) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. NIH-3T3, B. JC, C. BCL-1, D. C2C12.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CAS was performed by separating 30 µg of whole cell lysates by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CAS Polyclonal Antibody (Product # PA5-21468) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. PC-12, B. Rat2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CAS was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CAS Polyclonal Antibody (Product # PA5-21468) diluted at 1:2000. Red: phalloidin, a cytoskeleton marker.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CAS Polyclonal Antibody detects CSE1L protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CSE1L stained by CAS Polyclonal Antibody (Product # PA5-21468) diluted at 1:1500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using CSE1L (Product # PA5-21468) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.